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Given the massive wildfire incidences in Botswana and their essential negative and positive roles on ecosystem functions, this study aims to assess: (i) the immediate short-term impacts of wildfire on vegetation recovery, and (ii) the subsequent impact of wildfire and vegetation on soil biochemical properties (including pH, EC, total organic matter content, available P, and exchangeable cations (K, Mg, Ca, and Na)), as well as community level physiological profiling of the microbial community in the bulk and rhizosphere soil of dominant vegetation in the savanna ecosystem of Botswana